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使用抗碱性磷酸酶-抗碱性磷酸酶复合物（APAAP）、抗生物素蛋白-生物素复合物（ABC）和过氧化物酶抗过氧化物酶（PAP）免疫检测系统对妊娠子宫内膜生物素核内包涵体进行比较分析。

Comparative analysis of biotin intranuclear inclusions of gestational endometrium using the APAAP, ABC and the PAP immunodetection systems.

作者信息

Cooper K, Haffajee Z, Taylor L

机构信息

Department of Anatomical Pathology, School of Pathology, University of Witwatersrand, South Africa.

出版信息

J Clin Pathol. 1997 Feb;50(2):153-6. doi: 10.1136/jcp.50.2.153.

DOI:10.1136/jcp.50.2.153
PMID:9155698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499742/
Abstract

AIMS/BACKGROUND: Intranuclear inclusions have been observed in gestational endometrial glands. Although resembling Herpes simplex virus infected cells, these nuclei have been shown to contain endogenous biotin. Hence, immunohistochemical systems using the avidin-biotin complex will result in cross reaction and false positivity. This study investigates a series of 10 gestational endometria (formalin fixed and paraffin wax embedded) with intranuclear inclusions using three different immunodetection systems with primary anti-biotin.

RESULTS

Both the alkaline phosphatase anti-alkaline phosphatase (APAAP) and peroxidase anti-peroxidase (PAP) systems confirmed that the intranuclear inclusions were endogenous biotin. The streptavidin biotin complex (StreptABC) system produced a positive reaction in both test sections and controls (omitting primary anti-biotin) in the presence of prior blocking with free avidin and biotin. In addition, aberrant immunoreactivity was observed in adjacent nuclei/cytoplasm of endometrial glands and decidualised stroma in the negative control of the PAP system. This was subsequently eliminated using microwave pretreatment prior to immunohistochemistry.

CONCLUSION

The use of either the APAAP or PAP immunodetection systems (the latter with microwave pretreatment) is recommended for any immunohistochemical or non-isotopic in situ hybridisation investigation undertaken on gestational endometria.

摘要

目的/背景：在妊娠子宫内膜腺体中观察到核内包涵体。尽管这些细胞核类似于单纯疱疹病毒感染的细胞，但已证明它们含有内源性生物素。因此，使用抗生物素蛋白-生物素复合物的免疫组织化学系统会导致交叉反应和假阳性。本研究使用三种不同的带有抗生物素原的免疫检测系统，对一系列10例含有核内包涵体的妊娠子宫内膜（福尔马林固定、石蜡包埋）进行了研究。

结果

碱性磷酸酶抗碱性磷酸酶（APAAP）和过氧化物酶抗过氧化物酶（PAP）系统均证实核内包涵体为内源性生物素。在先用游离抗生物素蛋白和生物素封闭的情况下，链霉抗生物素蛋白-生物素复合物（StreptABC）系统在测试切片和对照（省略抗生物素原）中均产生阳性反应。此外，在PAP系统的阴性对照中，在子宫内膜腺体和蜕膜化基质的相邻细胞核/细胞质中观察到异常免疫反应性。随后在免疫组织化学之前使用微波预处理将其消除。

结论

对于对妊娠子宫内膜进行的任何免疫组织化学或非同位素原位杂交研究，建议使用APAAP或PAP免疫检测系统（后者采用微波预处理）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/be4eacad6d66/jclinpath00251-0067-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/6eb190b36c6c/jclinpath00251-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/8681c0a296b6/jclinpath00251-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/be4eacad6d66/jclinpath00251-0067-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/6eb190b36c6c/jclinpath00251-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/8681c0a296b6/jclinpath00251-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9150/499742/be4eacad6d66/jclinpath00251-0067-b.jpg

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本文引用的文献

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Tissue and intracellular distribution of biotin-C-1400H in rats and chicks.生物素-C-1400H在大鼠和雏鸡体内的组织及细胞内分布
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Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium.妊娠和产褥期子宫内膜腺体中含生物素的核内包涵体。
Am J Clin Pathol. 1993 Jan;99(1):13-7. doi: 10.1093/ajcp/99.1.13.
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Anomalous immunostaining of 'optically clear' nuclei in gestational endometrium. A potential pitfall in the diagnosis of pregnancy-related herpesvirus infection.
妊娠子宫内膜中“光学透明”细胞核的异常免疫染色。妊娠相关疱疹病毒感染诊断中的一个潜在陷阱。
Arch Pathol Lab Med. 1994 Aug;118(8):831-3.
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