Takayama K, Keith A D, Snipes W
Antimicrob Agents Chemother. 1975 Jan;7(1):22-4. doi: 10.1128/AAC.7.1.22.
The effect of isoniazid on the protoplasmic viscosity in the H37Ra strain of Mycobacterium tuberculosis was determined by using electron spin resonance spectroscopy and a small spin label tempone (2,2,6,6-tetramethylpiperidone-N-oxyl radical). Isoniazid (0.5 mug/ml) caused the internal cellular viscosity to increase gradually over the first 15 h of exposure from a rotational correlation time value (T(c)) of 2.4 x 10(-10) to 3.4 x 10(10) s and then decrease linearly to the control level after 27 h. These results could be interpreted to mean that isoniazid allows a continued and normal synthesis of the protoplasmic components while the rate of increase in the cell volume is reduced. A degradative process may begin after the initial 15-h exposure time, which would cause the reduction in the internal viscosity.
通过电子自旋共振光谱法和一种小的自旋标记物tempone（2,2,6,6 - 四甲基哌啶 - N - 氧基自由基），测定了异烟肼对结核分枝杆菌H37Ra菌株原生质粘度的影响。异烟肼（0.5微克/毫升）使细胞内粘度在暴露的前15小时内从旋转相关时间值（T(c)）2.4×10⁻¹⁰秒逐渐增加到3.4×10⁻¹⁰秒，然后在27小时后线性下降至对照水平。这些结果可以解释为异烟肼在细胞体积增加速率降低的同时，允许原生质成分持续正常合成。在最初暴露15小时后可能开始一个降解过程，这会导致内部粘度降低。
