Henry S A, Keith A D, Snipes W
Biophys J. 1976 Jun;16(6):641-53. doi: 10.1016/S0006-3495(76)85718-9.
Yeast mutants lacking fatty acid synthetase activity (fas-) die when deprived of saturated fatty acid under conditions which are otherwise growth-supporting. The spin label technique is used to show that restriction of molecular rotational diffusion of spin label molecules dissolved in aqueous zones increases several fold under conditions of fatty acid starvation while the apparent physical state of cellular hydrocarbon zones remains essentially unchanged. We focus attention on the cellular aqueous interior as the potential site of alteration under selective starvation conditions. Correspondences exist between restriction of molecular motion of water soluble spin labels dissolved in the cell and loss of cell viability. The correspondences to changes in the molecular motion of hydrocarbon soluble spin labels are much less or are not detectable.
缺乏脂肪酸合成酶活性的酵母突变体（fas-）在被剥夺饱和脂肪酸时，在其他条件均有利于生长的情况下会死亡。自旋标记技术用于表明，在脂肪酸饥饿条件下，溶解于水性区域的自旋标记分子的分子旋转扩散受限增加了几倍，而细胞烃类区域的表观物理状态基本保持不变。我们将注意力集中在细胞水性内部，认为其是选择性饥饿条件下可能发生改变的部位。溶解于细胞内的水溶性自旋标记分子的分子运动受限与细胞活力丧失之间存在对应关系。与烃溶性自旋标记分子运动变化的对应关系则少得多或无法检测到。
