Firth N A, Reade P C
Oral Medicine and Surgery Research Group, School of Dental Science, University of Melbourne.
Aust Dent J. 1996 Apr;41(2):83-6. doi: 10.1111/j.1834-7819.1996.tb05918.x.
Changes in the distribution of basement membrane components have been described in dysplastic lesions and in oral mucosal squamous cell carcinomas (OMSCC). The purpose of this study was to determine if these changes were related to pathological grade and if so, whether this had prognostic implications. Fifty formalin-fixed, paraffin-embedded specimens of OMSCC, with five or more years clinical follow-up, were studied using an immunoperoxidase technique for the detection of the basement membrane components, laminin and type IV collagen. The immunoreactivity of each component was evaluated and semiquantitatively scored as minimal, moderate or extensive and the results compared with the tumour size, node involvement and metastasis (TNM) clinical staging system and histopathological features. OMSCC were characterized by minimal or moderate staining with small islands of neoplastic cells frequently lacking staining for laminin and type IV collagen. Deposition of these components decreased with increased histopathological grade and absence of staining was more commonly associated with a poor prognosis. In particular the pattern of type IV collagen staining frequently differed from laminin staining. Neither of these parameters offered an advantage over TNM clinical staging with regard to prognosis. It was concluded that variations in laminin and type IV collagen immunoreactivity occurred in OMSCC and that high histopathological grade tumours with considerably diminished staining with anti-laminin and anti-type IV collagen carried a poor prognosis.
发育异常病变和口腔黏膜鳞状细胞癌（OMSCC）中已描述了基底膜成分分布的变化。本研究的目的是确定这些变化是否与病理分级相关，如果相关，这是否具有预后意义。使用免疫过氧化物酶技术检测基底膜成分层粘连蛋白和IV型胶原，对50例经过福尔马林固定、石蜡包埋且有5年或更长时间临床随访的OMSCC标本进行了研究。评估了每种成分的免疫反应性，并进行半定量评分，分为轻度、中度或广泛，然后将结果与肿瘤大小、淋巴结受累情况和转移（TNM）临床分期系统以及组织病理学特征进行比较。OMSCC的特征是染色为轻度或中度，肿瘤细胞小岛经常缺乏层粘连蛋白和IV型胶原染色。这些成分的沉积随着组织病理学分级的增加而减少，无染色更常与预后不良相关。特别是IV型胶原的染色模式常常与层粘连蛋白染色不同。就预后而言，这两个参数均未显示出优于TNM临床分期的优势。得出的结论是，OMSCC中存在层粘连蛋白和IV型胶原免疫反应性的变化，并且抗层粘连蛋白和抗IV型胶原染色明显减少的高组织病理学分级肿瘤预后不良。
