Adams W R, Green M H
Nutrition Department, Pennsylvania State University, University Park 16802.
J Nutr. 1994 Aug;124(8):1265-70. doi: 10.1093/jn/124.8.1265.
Male Sprague-Dawley rats (n = 41) that had liver vitamin A levels ranging from 5 nmol to 22 mumol (0.48 to 1629 nmol/g) were intubated with [3H]retinol in oil (167 kBq/rat). Plasma samples collected at 3, 4, 4.4, 5, 5.4 and 6 d were analyzed for radioactivity, and liver vitamin A mass was determined at 8 d. Nonlinear regression analysis was used to develop a biexponential equation describing the relationship at each sampling time between fraction of the dose remaining in plasma (FDp) and liver vitamin A. The best equation, developed with 3-d data, was liver vitamin A (nmol) = 58,577 x exp(-2715 x FDp) + 1810 x exp(-127 x FDp); Spearman's rank correlation coefficient = 0.982. This equation showed good predictive ability as evidenced by the ratio of the calculated to the observed liver vitamin A (1.23 +/- 0.65) and by its ability to distinguish animals with deficient, marginal, adequate or high vitamin A status. We conclude that this sensitive, noninvasive procedure can be used to predict rat liver vitamin A over a wide range.
雄性斯普拉格-道利大鼠（n = 41），其肝脏维生素A水平在5 nmol至22 μmol（0.48至1629 nmol/g）之间，经口灌胃给予油剂形式的[3H]视黄醇（167 kBq/只大鼠）。在3、4、4.4、5、5.4和6天时采集血浆样本分析放射性，并在8天时测定肝脏维生素A总量。采用非线性回归分析建立双指数方程，描述每个采样时间点血浆中剩余剂量分数（FDp）与肝脏维生素A之间的关系。用3天的数据建立的最佳方程为：肝脏维生素A（nmol）= 58577×exp（-2715×FDp）+ 1810×exp（-127×FDp）；斯皮尔曼等级相关系数 = 0.982。该方程具有良好的预测能力，计算得到的肝脏维生素A与观察值的比值为1.23±0.65，且能够区分维生素A缺乏、边缘、充足或高水平状态的动物。我们得出结论，这种灵敏的非侵入性方法可用于在较宽范围内预测大鼠肝脏维生素A水平。
