Furr H C, Amedee-Manesme O, Clifford A J, Bergen H R, Jones A D, Anderson D P, Olson J A
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Am J Clin Nutr. 1989 Apr;49(4):713-6. doi: 10.1093/ajcn/49.4.713.
The vitamin A status in 11 generally healthy surgical patients was estimated by measuring the dilution of a 45-mg oral dose of tetradeuterated retinyl acetate (99% pure). After purification of retinol by high-performance liquid chromatography, the ratio of 2H4-retinol:1H-retinol in plasma was measured by gas chromatography-mass spectrometry. On the basis of the observed ratios of [2H4]retinol:[1H]retinol over 19-47 d, the total body reserves and liver concentrations of vitamin A were calculated. Liver biopsy samples taken at surgery were directly analyzed for vitamin A. The correlation coefficient between calculated and measured liver vitamin A concentrations for 10 of the subjects was 0.88, and the Spearman's rank correlation coefficient was 0.95 (p less than 0.002). Thus, total body reserves of vitamin A in humans can be estimated validly in the marginal and satisfactory ranges by a benign, relatively noninvasive procedure.
通过测量口服45毫克四氘代视黄醇醋酸酯（99%纯）的稀释情况，对11名一般健康的外科手术患者的维生素A状况进行了评估。通过高效液相色谱法纯化视黄醇后，采用气相色谱-质谱法测定血浆中2H4-视黄醇:1H-视黄醇的比例。根据19至47天内观察到的[2H4]视黄醇:[1H]视黄醇比例，计算维生素A的全身储备量和肝脏浓度。手术时采集的肝脏活检样本直接用于维生素A分析。10名受试者计算得出的肝脏维生素A浓度与测量值之间的相关系数为0.88，Spearman等级相关系数为0.95（p小于0.002）。因此，通过一种良性、相对非侵入性的程序，可以在边缘和满意范围内有效估计人体维生素A的全身储备量。
