Hirata Y
Department of Otolaryngology, Ehime University, School of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1994 Jul;97(7):1191-9. doi: 10.3950/jibiinkoka.97.1191.
The author succeeded in producing vestibular ganglionitis by inoculating herpes simplex virus type 1 (HSV-1) into the back of the auricle of a new-born mouse. Postural deviation to the inoculated side was observed in 14 of 275 mice (5.1%) 6 to 10 days after the inoculation. In 6 of the 14 mice (42.9%) with postural deviation, HSV antigens were demonstrated immunohistochemically in the vestibular ganglion cells of the inoculated side. No antigens were identified in the animals with normal posture or in controls. Vestibular functions were evaluated in 5 animals suffering from postural deviation by the following test procedures. 1. behavior in an open field, 2. righting reflex, 3. transversing a narrow path, 4. ascending a rope, 5. negative geotaxis, 6. grasping a rod, 7. swimming. None could perform these tasks due to impairment of vestibular function. Histopathological and immunohistochemical examinations showed degeneration of the vestibular ganglion cells in 4 of the 5 mice, while HSV-1 antigens were recognized in 2 of them. The results of the present study show that inoculation of HSV into the back of the auricle of a new-born mouse can cause infection of the vestibular ganglion resulting in symptoms similar to those of vestibular neuritis, though the incidence is low compared to that of facial paralysis.
作者通过将1型单纯疱疹病毒（HSV-1）接种到新生小鼠耳廓后部成功诱发了前庭神经炎。接种后6至10天，275只小鼠中有14只（5.1%）出现向接种侧的姿势偏差。在14只出现姿势偏差的小鼠中，有6只（42.9%）通过免疫组织化学法在接种侧的前庭神经节细胞中检测到HSV抗原。姿势正常的动物或对照组中未发现抗原。通过以下测试程序对5只出现姿势偏差的动物进行了前庭功能评估。1. 在开阔场地中的行为，2. 翻正反射，3. 穿越狭窄路径，4. 攀爬绳索，5. 负趋地性，6. 抓杆，7. 游泳。由于前庭功能受损，这些动物均无法完成这些任务。组织病理学和免疫组织化学检查显示，5只小鼠中有4只的前庭神经节细胞发生退化，其中2只检测到HSV-1抗原。本研究结果表明，将HSV接种到新生小鼠耳廓后部可导致前庭神经节感染，产生与前庭神经炎相似的症状，尽管与面瘫相比发病率较低。
