Furuta Y, Takasu T, Fukuda S, Inuyama Y, Sato K C, Nagashima K
Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Acta Otolaryngol Suppl. 1993;503:85-9. doi: 10.3109/00016489309128081.
Viral infection has been considered to be a possible pathogenesis of vestibular neuronitis, and reactivation of the herpes simplex virus (HSV) is one of the most likely causes. However, it remains unknown whether the human vestibular ganglia contain latent HSV. We examined 26 vestibular ganglia from autopsied adults in search of HSV type 1 (HSV-1). To detect HSV-1, we used polymerase chain reaction (PCR), in situ hybridization and immunohistochemical staining. HSV DNA was detected in 6 of 10 vestibular ganglia using the PCR method. However, the latency-associated transcript (LAT) of HSV-1 was negative in all of the 16 vestibular ganglia examined. No HSV antigen was detected in any of the ganglia. These results indicate that HSV-1 is latently infected in the human vestibular ganglia, and that LAT is transcribed weakly or not at all.
病毒感染被认为是前庭神经炎可能的发病机制，单纯疱疹病毒（HSV）再激活是最可能的病因之一。然而，人类前庭神经节是否含有潜伏性HSV仍不清楚。我们检查了26例成年尸检者的前庭神经节以寻找1型单纯疱疹病毒（HSV-1）。为检测HSV-1，我们采用了聚合酶链反应（PCR）、原位杂交和免疫组织化学染色。用PCR方法在10个前庭神经节中的6个检测到了HSV DNA。然而，在所有检查的16个前庭神经节中，HSV-1的潜伏相关转录本（LAT）均为阴性。在任何神经节中均未检测到HSV抗原。这些结果表明，HSV-1潜伏感染于人类前庭神经节，且LAT转录微弱或根本不转录。
