Takenaka T, Kuribayashi K, Tsukiyama M, Nakamine H, Maeda J, Hiroi K, Kuno T, Nakamura Y
Department of Laboratory Medicine, Wakayama Medical School.
Rinsho Byori. 1994 Jul;42(7):725-30.
Using polymerase chain reaction (PCR) amplification and Southern hybridization, gag, pol, env and pX region genes of HTLV-1 provirus were detected in T-cell malignancy such as adult T-cell leukemia/lymphoma (ATLL), mycosis fungoides in leukemic phase (MF) and, CD8-leukemia. The gag, pol, and/or env regions, were not amplified in some cases of ATLL, which was considered to be induced by mutation, but not deletion. However, the pX-1 and pX-2 regions could be amplified in all cases examined. As it is suggested that the pX gene plays an important role in leukemogenesis, the mutation may not occur in this region gene. Interestingly, the pX-2 was amplified in the cases with MF and CD8-leukemia as well. The amplified products were hybridized to the HTLV-1 pX sequence, even though the products contained DNA fragments with a size larger than expected as well as those of an expected size. These results indicated the possibility that the virus having sequence identical with HTLV-1 pX is integrated in the tumor cells of MF and CD8 leukemia.
利用聚合酶链反应（PCR）扩增和Southern杂交技术，在成人T细胞白血病/淋巴瘤（ATLL）、白血病期蕈样肉芽肿（MF）和CD8白血病等T细胞恶性肿瘤中检测到了HTLV-1前病毒的gag、pol、env和pX区域基因。在某些ATLL病例中，gag、pol和/或env区域未被扩增，这被认为是由突变而非缺失引起的。然而，在所有检测病例中，pX-1和pX-2区域均可被扩增。由于提示pX基因在白血病发生中起重要作用，该区域基因可能未发生突变。有趣的是，在MF和CD8白血病病例中也扩增出了pX-2。尽管扩增产物包含大小大于预期的DNA片段以及预期大小的片段，但这些产物与HTLV-1 pX序列杂交。这些结果表明，与HTLV-1 pX序列相同的病毒可能整合在MF和CD8白血病的肿瘤细胞中。
