Chan W C, Hooper C, Wickert R, Benson J M, Vardiman J, Hinrichs S, Weisenburger D
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-3135.
Diagn Mol Pathol. 1993 Sep;2(3):192-9.
Several recent studies reported the detection of partially deleted HTLV-I provirus in biopsies of lesions from patients with mycosis fungoides (MF) and T-cell anaplastic large-cell lymphoma. We studied lesions from 59 patients (21 B-cell lymphomas: 16 diffuse and five follicular; 11 cutaneous T-cell lymphomas, including seven MF; one T-immunoblastic lymphoma; 10 diffuse anaplastic large-cell lymphomas: two B, four T, and four of indeterminate phenotype; three Hodgkin's lymphomas; eight atypical lymphoid proliferations; four other lymphoid lesions, and one squamous-cell carcinoma) using primers to the gag, pol and pX regions of HTLV-I in the polymerase chain reaction (PCR) to detect relevant sequences. A total of 10 patients showed one or more PCR-amplifiable products, including five of 11 patients with cutaneous T-cell lymphomas (45%) as compared with one of 21 patients with B-cell lymphomas (4.3%). We did not find a high incidence of positivity in anaplastic large-cell lymphomas, as reported previously. Detectable HTLV-I sequences were not limited to any subtype of lymphoma, and a pX sequence was detected in a squamous-cell carcinoma. Sequence analysis of one amplified product from each of the three regions studied showed a 94.2, 100, and 98.9% homology to the corresponding prototypical gag, pol, and pX HTLV-I sequences, respectively, indicating that the amplified sequences were derived from HTLV-I or a very closely related virus. HTLV-I sequences were detected in a significant proportion of patients with cutaneous T-cell lymphoma, but their role in the pathogenesis of the neoplasm is still unclear.
最近的几项研究报告称，在蕈样肉芽肿（MF）和T细胞间变性大细胞淋巴瘤患者的病变活检中检测到部分缺失的HTLV-I前病毒。我们使用针对HTLV-I的gag、pol和pX区域的引物，通过聚合酶链反应（PCR）研究了59例患者的病变（21例B细胞淋巴瘤：16例弥漫性和5例滤泡性；11例皮肤T细胞淋巴瘤，包括7例MF；1例T免疫母细胞淋巴瘤；10例弥漫性间变性大细胞淋巴瘤：2例B细胞型、4例T细胞型和4例表型不确定；3例霍奇金淋巴瘤；8例非典型淋巴样增生；4例其他淋巴样病变，以及1例鳞状细胞癌），以检测相关序列。共有10例患者显示出一种或多种可通过PCR扩增的产物；11例皮肤T细胞淋巴瘤患者中有5例（45%）出现这种情况，相比之下，21例B细胞淋巴瘤患者中只有1例（4.3%）出现。我们没有发现间变性大细胞淋巴瘤中如先前报道的高阳性率。可检测到的HTLV-I序列不限于任何淋巴瘤亚型，并且在一例鳞状细胞癌中检测到了pX序列。对所研究的三个区域中每个区域的一个扩增产物进行序列分析，结果显示分别与相应的原型gag、pol和pX HTLV-I序列具有94.2%、100%和98.9%的同源性，这表明扩增序列源自HTLV-I或一种密切相关的病毒。在相当一部分皮肤T细胞淋巴瘤患者中检测到了HTLV-I序列，但其在肿瘤发病机制中的作用仍不清楚。
