Lekholm U, Wallenius K, Heyden G
Histochemistry. 1975 Jul 30;44(2):147-54. doi: 10.1007/BF00494076.
The hard palates of 150 female albino rats of the Sprague-Dawley strain were painted 3 times a week either with the fat-soluble carcinogen 7,12-dimethylbenz(a)anthracene (DMBA) for 11 months with inhibited secretion of saliva or with the water-soluble carcinogen 4-nitrochinoline N-oxide (4NQO) for 8 months with intact salivary secretion. Specimens were taken regularly from the mucosa of the hard palate, and the content of lipids in the epithelium was studied histochemically and biochemically during the carcinogenesis. Changes in the lipid content could be observed histochemically as there was a focal loss of lipid stainability in the epithelium during the more advanced stages of carcinogenesis with severe epithelial dysplasia, carcinoma in situ and early invasive carcinoma. The biochemical method used (TLC) could not, however, verify the histologically observed changes in the lipid content of the epithelium, probably because the changes were very local.
每周三次用脂溶性致癌物7,12-二甲基苯并(a)蒽（DMBA）对150只Sprague-Dawley品系的雌性白化大鼠的硬腭进行涂抹，持续11个月，期间唾液分泌受到抑制；或者每周三次用水溶性致癌物4-硝基喹啉-N-氧化物（4NQO）对其硬腭进行涂抹，持续8个月，期间唾液分泌正常。定期从硬腭黏膜取材，在致癌过程中对上皮组织中的脂质含量进行组织化学和生物化学研究。在致癌过程的更晚期，即出现严重上皮发育异常、原位癌和早期浸润癌时，组织化学观察到上皮组织中脂质染色性出现局灶性丧失，提示脂质含量发生了变化。然而，所采用的生化方法（薄层色谱法）无法证实组织学观察到的上皮组织脂质含量变化，可能是因为这些变化非常局限。
