Yamasaki M, Kumazawa M, Kohsaka T, Nakamura H
Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Oral Surg Oral Med Oral Pathol. 1994 Jun;77(6):655-61. doi: 10.1016/0030-4220(94)90330-1.
We investigated the effect of methotrexate-induced neutropenia on periapical lesions in rats. Periapical lesions were prepared in all animals by pulpal exposure. The animals were injected with methotrexate three times a week 4 weeks before or after the pulpal exposure. The periapical lesion of each animal was examined histologically, histometrically, and immunohistochemically. The total leukocyte count and neutrophil count in the peripheral blood decreased after the injection. The methotrexate-induced neutropenia elicited after pulpal exposure did not change the status of the periapical lesion, whereas the neutropenia before the exposure inhibited the development of the lesion. Moreover, the neutropenia before pulpal exposure resulted in an inhibition of infiltration of neutrophils into the lesion.
我们研究了甲氨蝶呤诱导的中性粒细胞减少对大鼠根尖周病变的影响。通过牙髓暴露在所有动物中制备根尖周病变。在牙髓暴露前或暴露后4周，每周给动物注射3次甲氨蝶呤。对每只动物的根尖周病变进行组织学、组织计量学和免疫组织化学检查。注射后外周血白细胞总数和中性粒细胞计数下降。牙髓暴露后诱发的甲氨蝶呤诱导的中性粒细胞减少并未改变根尖周病变的状态，而暴露前的中性粒细胞减少则抑制了病变的发展。此外，牙髓暴露前的中性粒细胞减少导致中性粒细胞向病变部位浸润受到抑制。
