一种来自粟酒裂殖酵母的新型不依赖ATP的DNA内切核酸酶，它能识别环丁烷嘧啶二聚体和6-4光产物。

A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6-4 photoproducts.

作者信息

Bowman K K, Sidik K, Smith C A, Taylor J S, Doetsch P W, Freyer G A

机构信息

Department of Biochemistry, Emory University School of Medicine, Rollins Research Center, Atlanta, GA 30322.

出版信息

Nucleic Acids Res. 1994 Aug 11;22(15):3026-32. doi: 10.1093/nar/22.15.3026.

We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.