Davey S, Nass M L, Ferrer J V, Sidik K, Eisenberger A, Mitchell D L, Freyer G A
Cancer Research Laboratories, Queen's University, Kingston, Canada K7L 3N6.
Nucleic Acids Res. 1997 Mar 1;25(5):1002-8. doi: 10.1093/nar/25.5.1002.
In addition to nucleotide excision repair (NER), the fission yeast Schizosaccharomyces pombe possesses a UV damage endonuclease (UVDE) for the excision of cyclobutane pyrimidine dimers and 6-4 pyrimidine pyrimidones. We have previously described UVDE as part of an alternative excision repair pathway, UVDR, for UV damage repair. The existence of two excision repair processes has long been postulated to exist in S.pombe, as NER-deficient mutants are still proficient in the excision of UV photoproducts. UVDE recognizes the phosphodiester bond immediately 5'of the UV photoproducts as the initiating event in this process. We show here that UVDE activity is inducible at both the level of uve1+ mRNA and UVDE enzyme activity. Further, we show that UVDE activity is regulated by the product of the rad12 gene.
除了核苷酸切除修复（NER）外，裂殖酵母粟酒裂殖酵母还拥有一种紫外线损伤内切核酸酶（UVDE），用于切除环丁烷嘧啶二聚体和6-4嘧啶嘧啶酮。我们之前曾将UVDE描述为紫外线损伤修复的替代切除修复途径UVDR的一部分。长期以来，人们一直推测粟酒裂殖酵母中存在两种切除修复过程，因为NER缺陷型突变体在切除紫外线光产物方面仍然 proficient。UVDE将紫外线光产物5'端紧邻的磷酸二酯键识别为该过程中的起始事件。我们在此表明，UVDE活性在uve1+ mRNA水平和UVDE酶活性水平均可诱导。此外，我们表明UVDE活性受rad12基因产物的调节。
