Baumgartner S, Martin D, Hagios C, Chiquet-Ehrismann R
Friedrich Miescher-Institut, Basel, Switzerland.
EMBO J. 1994 Aug 15;13(16):3728-40. doi: 10.1002/j.1460-2075.1994.tb06682.x.
We describe the molecular characterization of the Drosophila gene tenm, a large transcription unit spanning > 110 kb of DNA. tenm encodes a large extracellular protein of 2515 amino acids related to the extracellular matrix molecule tenascin. The Tenm protein is found in seven stripes during the blastoderm stage, and each stripe overlaps with the even-skipped stripes. tenm mutants show a phenotype resembling that of odd-paired (opa), a member of the pair-rule class of segmentation genes. Thus, Tenm is the first example of a pair-rule gene product acting from outside the cell. While the Tenm protein is under the control of fushi tarazu and even-skipped, but not of opa, at least two pair-rule genes, paired (prd) and sloppy paired (slp), and all segment-polarity genes analysed to date are under the control of tenm. Our data suggest that Tenm initiates a signal transduction cascade which acts, via or in concert with opa, on downstream targets such as prd, slp, gooseberry, engrailed and wingless, leading to an opa-like phenotype.
我们描述了果蝇基因tenm的分子特征，它是一个跨越超过110 kb DNA的大型转录单位。tenm编码一种由2515个氨基酸组成的大型细胞外蛋白，与细胞外基质分子腱生蛋白相关。在胚盘阶段，Tenm蛋白出现在七条条纹中，且每条条纹与偶数跳读条纹重叠。tenm突变体表现出一种类似于奇数配对（opa）的表型，opa是配对规则类分割基因的成员。因此，Tenm是第一个从细胞外起作用的配对规则基因产物的例子。虽然Tenm蛋白受分节基因和偶数跳读的控制，但不受opa的控制，至少两个配对规则基因，配对（prd）和草率配对（slp），以及迄今为止分析的所有体节极性基因都受tenm的控制。我们的数据表明，Tenm启动了一个信号转导级联反应，该反应通过opa或与opa协同作用于下游靶点，如prd、slp、醋栗、engrailed和无翅基因，从而导致类似opa的表型。
