Hatala M, Prát V, Liska M, Konícková L, Slugen I
Zentralbl Bakteriol Orig A. 1975;230(4):466-9.
The model of experimental pyocyanic renal infection in rats based on direct inoculation of bacteria to the renal parenchyma is described. The authors describe in detail the procedure and evaluation of the results with respect to microbiology, histology and reproducibility. The method produced a focal supurrative inflammation with a significant bacteriologica finding in renal tissue for 8 weeks at the least. The inflammation has a tendency to form focal cicatrices not accompanied by diffuse shrinkage of the kidney. The method is well reproducible and its technique is relatively simple thus permitting to induce infection in a large series of animals within a short period of time. In the second part of the paper, the use of this method in testing the therapeutical efficacy of gentamicin, carbenicillin and their combination is reported. The results showed gentamicin to be more effective than carbenicillin and the combination of the two to have the highest therapeutical effect. Moreover, their combination made it possible to reduce the dosage of the antibiotics and thereby reduce their potential undesirable effect. Further, the results showed the described model of experimental renal infection to be well suited for testing the efficacy of chemotherapeutics and resolving some problems of the treatment of infectious diseases.
本文描述了一种通过将细菌直接接种到大鼠肾实质来建立实验性绿脓杆菌肾感染的模型。作者详细介绍了该模型在微生物学、组织学及可重复性方面的操作步骤和结果评估。该方法至少在8周内可引发局灶性化脓性炎症，并在肾组织中出现显著的细菌学发现。炎症有形成局灶性瘢痕的倾向，且不伴有肾脏弥漫性萎缩。该方法具有良好的可重复性，技术相对简单，因此能够在短时间内对大量动物诱发感染。在论文的第二部分，报告了该方法在测试庆大霉素、羧苄青霉素及其联合用药治疗效果方面的应用。结果表明，庆大霉素比羧苄青霉素更有效，二者联合使用治疗效果最佳。此外，联合用药能够降低抗生素剂量，从而减少其潜在的不良影响。此外，结果还表明，所描述的实验性肾感染模型非常适合测试化疗药物的疗效，并解决传染病治疗中的一些问题。
