Nishi T, Tsuchiya K
Infect Immun. 1978 Nov;22(2):508-15. doi: 10.1128/iai.22.2.508-515.1978.
Urinary tract infection with Pseudomonas aeruginosa was induced in mice by transurethral inoculation of the organism into the bladder, followed by urethral obstruction for 6 h. The infection was mostly localized in the urinary organs. P. aeruginosa P9 was selected as the challenge organism from 10 laboratory strains of P. aeruginosa. After the inoculation of 10(7) colony-forming units of P. aeruginosa P9, transient bacteremia was observed in some of the mice from 6 h to 1 day after the inoculation. The number of organisms in the bladder tissue gradually decreased, whereas that in the kidneys increased to levels of 10(6) to 10(7) colony-forming units in 3 days, and these levels remained up to 2 weeks after the inoculation. The organisms gradually disappeared thereafter, and spontaneous recovery took place. The organisms could be recovered from the kidneys of 95% of the mice, and the gross lesions in the kidneys were observed in 77% of the mice 1 week after inoculation. The method developed here is simple and may be useful in the study of urinary tract infections due to P. aeruginosa and other species of bacteria.20
通过将铜绿假单胞菌经尿道接种到小鼠膀胱，随后进行6小时的尿道梗阻，诱导小鼠发生铜绿假单胞菌引起的尿路感染。感染主要局限于泌尿器官。从10株铜绿假单胞菌实验室菌株中选择铜绿假单胞菌P9作为攻击菌。接种10⁷个铜绿假单胞菌P9菌落形成单位后，部分小鼠在接种后6小时至1天出现短暂菌血症。膀胱组织中的菌数逐渐减少，而肾脏中的菌数在3天内增加到10⁶至10⁷个菌落形成单位水平，且这些水平在接种后持续2周。此后菌数逐渐消失，小鼠自发恢复。95%的小鼠肾脏中可检出该菌，接种1周后77%的小鼠肾脏出现肉眼可见病变。这里建立的方法简单，可能有助于研究由铜绿假单胞菌和其他细菌引起的尿路感染。
