Ansorg R, Zappel H, Thomssen R
Zentralbl Bakteriol Orig A. 1975;230(4):492-507.
The disc agar-diffusion-test using Bacillus subtilis ATCC 6051 as test organism is a simple and rapid method for routine testing of antibacterial agents in urine specimens. The test records urine levels which are expected under medium dosage, and in many cases even lower concentrations of renal excreted antibiotics. Out of 5655 analysed urine samples 22% contain antibacterial substances. In urine specimens over which information was volunteered that either no chemotherapy had been administered or that more than a three day's interval free of therapy existed, inhibitory substances are found in 8% and 27% respectively. Urine specimens which are supposedly collected from patients under current chemotherapy do not show therapeutic relevant antibiotic levels in 26%. Between urine specimens with and without antibacterial activity there is no significant difference in the incidence of viable counts of 10-4-10-5/ml and 10-5/ml. From urine samples with antibacterial content increases in the numbers of multiple resistant strains of E. coli, Proteus spp., Pseudom. aerug. and Enterobacter spp. together with high numbers of Candida spp. are observed.
以枯草芽孢杆菌ATCC 6051作为测试菌的纸片琼脂扩散试验，是一种用于尿液标本中抗菌剂常规检测的简单快速方法。该试验记录的是中等剂量下预期的尿液浓度，在许多情况下甚至是肾脏排泄抗生素的更低浓度。在5655份分析的尿液样本中，22%含有抗菌物质。在那些主动提供信息表明未进行化疗或已停药超过三天的尿液标本中，分别有8%和27%发现了抑制物质。据推测从正在接受化疗的患者采集的尿液标本中，有26%未显示出与治疗相关的抗生素水平。在有和没有抗菌活性的尿液标本之间，每毫升10⁻⁴ - 10⁻⁵个活菌数和每毫升10⁻⁵个活菌数的发生率没有显著差异。在含有抗菌成分的尿液样本中，观察到大肠杆菌、变形杆菌属、铜绿假单胞菌和肠杆菌属的多重耐药菌株数量增加，同时念珠菌属数量也很多。
