Martinot-Peignoux M, Marcellin P, Gournay J, Gabriel F, Courtois F, Branger M, Wild A M, Erlinger S, Benhamou J P
Unité de Recherches de Physiopathologie Hépatique (INSERM), Service d'Hépatologie, Hôpital Beaujon, Clichy, France.
J Hepatol. 1994 May;20(5):676-8. doi: 10.1016/s0168-8278(05)80360-5.
The detection of serum HCV-RNA needs to be standardized. The aim of this study was to assess the effectiveness of the branched DNA amplification method in detecting and quantitating serum HCV-RNA in 54 blood donors, 33 with and 21 without increased serum alanine aminotransferase levels and with detectable serum HCV-RNA by polymerase chain reaction. HCV-RNA was detected by branched DNA signal amplification in 42/54 (77%) of the blood donors. Positivity rates were not different among the 21 blood donors with normal and the 33 blood donors with increased serum alanine aminotransferase levels (86% and 76%, respectively). Median serum HCV-RNA levels were not different among donors with or without increased serum alanine aminotransferase levels (28.6 x 10(5) Eq/ml and 14.7 x 10(5) Eq/ml, respectively). There was no significant correlation between serum alanine aminotransferase levels and serum HCV-RNA levels. These findings show that branched DNA signal amplification identifies most of the donors with true hepatitis C virus viremia and that the level of hepatitis C virus replication is not correlated to serum alanine aminotransferase levels.
血清丙型肝炎病毒核糖核酸（HCV-RNA）的检测需要标准化。本研究的目的是评估分支DNA扩增法在检测和定量54名献血者血清HCV-RNA中的有效性，其中33名献血者血清丙氨酸氨基转移酶水平升高，21名献血者血清丙氨酸氨基转移酶水平正常，且通过聚合酶链反应可检测到血清HCV-RNA。采用分支DNA信号扩增法在42/54（77%）的献血者中检测到HCV-RNA。血清丙氨酸氨基转移酶水平正常的21名献血者与血清丙氨酸氨基转移酶水平升高的33名献血者的阳性率无差异（分别为86%和76%）。血清丙氨酸氨基转移酶水平升高或正常的献血者之间，血清HCV-RNA水平中位数无差异（分别为28.6×10⁵Eq/ml和14.7×10⁵Eq/ml）。血清丙氨酸氨基转移酶水平与血清HCV-RNA水平之间无显著相关性。这些研究结果表明，分支DNA信号扩增法可识别出大多数真正丙型肝炎病毒血症的献血者，且丙型肝炎病毒复制水平与血清丙氨酸氨基转移酶水平无关。
