Zygulska-Mach H, Grabowska-Maślanka H, Janik A, Gawlik M
Katedry i Kliniki Okulistyki Collegium Medicum, Uniwersytetu Jagiellońskiego, Krakowie.
Klin Oczna. 1993 Nov-Dec;95(11-12):377-9.
The experiments were carried out on 21 rabbits, divided into two groups. The first group consisted of 10 control animals, the second one consisted of 11 animals given per os 3 times per week for 3 months 1 ml of water solution of cadmium chloride in the dose of 0.35 mg for 1 kilogram of body weight. After the animals have been killed, the content of cholesterol, triglycerides and phospholipids in their vitreous was identified with enzymatic methods, applying appropriate tests. The content of free fatty acids was estimated with the Duncombe method. The obtained results were analyzed statistically, with t-Student test. It was determined statistically that the content of all tested lipids components in the vitreous of rabbits poisoned with cadmium chloride was significantly decreased in comparison with their content in control animals. The greatest decrease was found in the level of free fatty acids and triglycerides, smaller decrease in phospholipids and cholesterol. Significant decrease of the total cholesterol level was found in the rabbits' lens. However no changes were observed in any other lipid components of the lens. The article discusses the significance of the obtained results in relation to ecological problems.
实验在21只兔子身上进行，分为两组。第一组由10只对照动物组成，第二组由11只动物组成，每周经口给予3次，持续3个月，剂量为每千克体重0.35毫克的1毫升氯化镉水溶液。动物处死后，采用酶法并应用适当的检测方法测定其玻璃体内胆固醇、甘油三酯和磷脂的含量。游离脂肪酸含量用邓科姆法估算。所得结果采用t检验进行统计学分析。经统计学测定，与对照动物相比，氯化镉中毒兔子玻璃体内所有检测脂质成分的含量均显著降低。游离脂肪酸和甘油三酯水平下降幅度最大，磷脂和胆固醇下降幅度较小。在兔子晶状体中发现总胆固醇水平显著下降。然而，晶状体的任何其他脂质成分均未观察到变化。本文讨论了所得结果与生态问题相关的意义。
