99锝-六甲基丙二胺肟与99锝-双半胱乙酯作为白细胞标记剂的对比研究。

A comparative study of 99Tcm-HMPAO and 99Tcm-ECD as a leukocyte labelling agent.

作者信息

Kao C H, Huang W T, Wang Y L, Lin K S, Lo J M, Wang S J

机构信息

Department of Nuclear Medicine, Taichung Veterans General Hospital, Taiwan, Republic of China.

出版信息

Nucl Med Commun. 1994 Apr;15(4):294-7. doi: 10.1097/00006231-199404000-00162.

DOI:10.1097/00006231-199404000-00162

PMID:8072744

Abstract

An attempt was made to use 99Tcm-ethyl cysteinate dimer (99Tcm-ECD) (Neurolite, Du Pont Merck, N. Billerica, MA) to label leukocytes. The radiochemical purity of 99Tcm-ECD, labelling efficiency of leukocytes, cell viability of labelled leukocytes and stability of 99Tcm-ECD-labelled leukocytes were calculated. Compared with the commercial cell-labelling agent, 99Tcm-hexamethylpropyleneamine oxime (99Tcm-HMPAO): (1) the radiochemical purity of 99Tcm-ECD was higher than that of 99Tcm-HMPAO and at immediately, 1, 2, 4, 6, 8 and 24 h after 99Tcm labelling; (2) the labelling efficiency of 99Tcm-ECD-labelled leukocytes was lower than that of 99Tcm-HMPAO; (3) the viability of the labelled white blood cells (WBC) was high for both agents; and (4) the stability of 99Tcm-ECD-labelled leukocytes was worse than that of 99Tcm-HMPAO at 1, 2, 4, 6, 8 and 24 h. It is concluded that although 99Tcm-ECD is more stable than 99Tcm-HMPAO, because of the lower labelling efficiency and power stability of 99Tcm-ECD-labelled leukocytes, 99Tcm-ECD is not a good choice as a leukocyte-labelling agent to replace commercial 99Tcm-HMPAO.

摘要

曾尝试使用99锝-乙基半胱氨酸二聚体（99Tcm-ECD）（Neurolite，杜邦默克公司，马萨诸塞州比勒里卡）标记白细胞。计算了99Tcm-ECD的放射化学纯度、白细胞标记效率、标记白细胞的细胞活力以及99Tcm-ECD标记白细胞的稳定性。与市售细胞标记剂99锝-六甲基丙烯胺肟（99Tcm-HMPAO）相比：（1）99Tcm-ECD在99Tcm标记后即刻、1、2、4、6、8和24小时的放射化学纯度高于99Tcm-HMPAO；（2）99Tcm-ECD标记白细胞的标记效率低于99Tcm-HMPAO；（3）两种试剂标记的白细胞（WBC）活力均较高；（4）99Tcm-ECD标记白细胞在1、2、4、6、8和24小时的稳定性比99Tcm-HMPAO差。结论是，尽管99Tcm-ECD比99Tcm-HMPAO更稳定，但由于99Tcm-ECD标记白细胞的标记效率较低且稳定性欠佳，99Tcm-ECD并非替代市售99Tcm-HMPAO作为白细胞标记剂的理想选择。