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[用单向辐射免疫扩散法测定血凝素含量对所用检测系统及去污剂的依赖性]

[The dependence of determining the hemagglutinin content by SRID on the test system used and on the detergent].

作者信息

Kastrikina L N, Lonskaia N I

出版信息

Vopr Virusol. 1993 May-Jun;38(3):132-5.

PMID:8073752
Abstract

The assay systems for single radial immunodiffusion (SRD) represented by standard reagents of antigens and monospecific antisera to A and B influenza virus haemagglutinin and prepared in two different countries were used for elucidation of the influence of monospecific antisera and some ionic and nonionic detergents on the determination of hemagglutinin content in the homologous (antigen and antiserum from the same country) and heterologous (antigen and antiserum from different countries) assay systems. A high specificity of SRD reaction excluding substitution of antiserum from one country for analogous preparation from another as well as detergent interchangeability were shown.

摘要

以A和B型流感病毒血凝素抗原标准试剂及单特异性抗血清为代表的单向辐射免疫扩散(SRD)检测系统,分别在两个不同国家制备,用于阐明单特异性抗血清以及一些离子型和非离子型去污剂对同源(来自同一国家的抗原和抗血清)和异源(来自不同国家的抗原和抗血清)检测系统中血凝素含量测定的影响。结果表明,SRD反应具有高特异性,排除了用一个国家的抗血清替代另一个国家类似制剂的可能性,同时也证明了去污剂的互换性。

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