pH值和离子强度对流感病毒血凝素定性检测中单向辐射免疫扩散试验的影响。
The influence of pH and ionic strength on the single radial immunodiffusion test in qualitative assay of influenza virus haemagglutinin.
作者信息
Willkommen H, Platen S, Stäber H
出版信息
Acta Virol. 1983 Sep;27(5):407-11.
PMID:6139944
Abstract
A significant dependence between different ionic strength and pH value of virus suspension on one hand and the haemagglutinin (HA) content as determined by single-radial-immunodiffusion (SRD) test on the other hand was observed after cleavage of influenza virus recombinant NIB-6 (H1N1) with sodium lauroyl sarcosinate (SLS). In contrast, no such relationship was found when the HA Of NIB-4 (H3N2) recombinant strain was determined.
摘要
在用月桂酰肌氨酸钠（SLS）裂解流感病毒重组体NIB - 6（H1N1）后，观察到一方面病毒悬液的不同离子强度和pH值与另一方面通过单向放射免疫扩散（SRD）试验测定的血凝素（HA）含量之间存在显著相关性。相比之下，在测定NIB - 4（H3N2）重组株的HA时未发现这种关系。
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