Bizhanov G I, Kastrikina L N, Lonskaya N I, Popov V F
L.A. Tarasevich State Institute of Standardization and Control of Biologie Preparations, U.S.S.R. Ministry of Health, Moscow.
Acta Virol. 1988 May;32(3):252-60.
The influence of ionic, nonionic and amphoteric detergents or surfactants (SA) on determination of influenza A virus haemagglutinin content in the inactivated influenza vaccine by single-radial-immunodiffusion technique has been investigated. It was found that the action of different SA on IIV in SRID test is dependent on the mode of vaccine production, the procedure of virus inactivation and probably also on the specific structure of the antigen tested. Nevertheless, for nearly all SA used in this work the optimal concentrations (largest precipitation area) ranged from 1 to 2%; lower concentrations (except of amphoteric detergents) failed to yield an optimal effect.
研究了离子型、非离子型和两性离子去污剂或表面活性剂（SA）对采用单向辐射免疫扩散技术测定灭活流感疫苗中甲型流感病毒血凝素含量的影响。结果发现，不同SA在单向辐射免疫扩散试验中对流感灭活疫苗的作用取决于疫苗生产方式、病毒灭活程序，可能还取决于所检测抗原的特定结构。然而，对于本研究中使用的几乎所有SA，最佳浓度（最大沉淀面积）范围为1%至2%；较低浓度（两性离子去污剂除外）未能产生最佳效果。
