Influence of detergents on measurement of influenza A virus haemagglutinin content in inactivated influenza vaccine by single radial immunodiffusion.

The influence of ionic, nonionic and amphoteric detergents or surfactants (SA) on determination of influenza A virus haemagglutinin content in the inactivated influenza vaccine by single-radial-immunodiffusion technique has been investigated. It was found that the action of different SA on IIV in SRID test is dependent on the mode of vaccine production, the procedure of virus inactivation and probably also on the specific structure of the antigen tested. Nevertheless, for nearly all SA used in this work the optimal concentrations (largest precipitation area) ranged from 1 to 2%; lower concentrations (except of amphoteric detergents) failed to yield an optimal effect.