Aono T, Murakami S, Yanagihara N, Yamanishi K
Department of Virology, Osaka University, Japan.
Acta Otolaryngol Suppl. 1994;514:132-4. doi: 10.3109/00016489409127577.
A simple and rapid diagnostic assay system was developed for detecting and identifying human alpha-herpesviruses, such as herpes simplex virus types 1 and 2 and varicella-zoster virus, by polymerase chain reaction. This system was based on an amplification step, using primers that bind the DNA polymerase consensus sequence of alpha-human herpesviruses, and a detection step, using non-radioactive virus-specific probes. This method could be used to amplify any human alpha-herpesviruses, and each virus-specific probe was highly specific for identification of the amplified product. This system is readily applicable for implementation in the clinical laboratory.
开发了一种简单快速的诊断检测系统，用于通过聚合酶链反应检测和鉴定人类α-疱疹病毒，如1型和2型单纯疱疹病毒以及水痘带状疱疹病毒。该系统基于一个扩增步骤，使用与人类α-疱疹病毒DNA聚合酶共有序列结合的引物，以及一个检测步骤，使用非放射性病毒特异性探针。该方法可用于扩增任何人类α-疱疹病毒，并且每种病毒特异性探针对于扩增产物的鉴定具有高度特异性。该系统易于应用于临床实验室。
