Wei S G, Wang L F, Miao S Y, Zong S D, Koide S S
Chinese Academy of Medical Sciences, Institute of Basic Medical Sciences, Beijing.
Arch Androl. 1994 May-Jun;32(3):251-62. doi: 10.3109/01485019408987793.
Serum obtained from an infertile subject possessed antibodies that interacted with a human sperm glycoprotein with an estimated M(r) of 17,550 and pI of 5.65 containing 17.7% neutral hexoses and designated as the BS-17 component. Polyclonal antibodies raised against the BS-17 antigen blocked the capacity of human sperm to fertilize zona-free hamster ova in vitro; however, the antibodies did not influence the binding of human sperm to zone-free ova or alter the motility of human sperm. The antibodies inhibited the capacity of mouse sperm to fertilize ova upon in vivo insemination. The BS-17 antigen was detected in human, rat, mouse, rabbit, and hamster sperm by an immunocytochemical method, using polyclonal anti-BS-17 antibodies. Intense staining occurred over the surface of the acrosomal region of all mammalian sperm. The results suggest that the production of anti-BS-17 antibodies contribute to infertility by preventing the capacitation of sperm and/or by blocking the ability of capacitated sperm to fertilize the egg.
从一名不孕不育患者体内获取的血清中含有抗体，这些抗体可与一种人类精子糖蛋白发生相互作用。该糖蛋白的估计相对分子质量为17,550，等电点为5.65，含有17.7%的中性己糖，被命名为BS-17成分。针对BS-17抗原产生的多克隆抗体在体外可阻断人类精子使去透明带仓鼠卵受精的能力；然而，这些抗体并不影响人类精子与去透明带卵的结合，也不改变人类精子的活力。这些抗体在体内授精时可抑制小鼠精子使卵子受精的能力。采用多克隆抗BS-17抗体，通过免疫细胞化学方法在人类、大鼠、小鼠、兔子和仓鼠的精子中检测到了BS-17抗原。在所有哺乳动物精子的顶体区域表面均出现强烈染色。结果表明，抗BS-17抗体的产生通过阻止精子获能和/或通过阻断获能精子使卵子受精的能力而导致不孕不育。
