Lee J Y, Koi M, Stanbridge E J, Oshimura M, Kumamoto A T, Feinberg A P
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109.
Nat Genet. 1994 May;7(1):29-33. doi: 10.1038/ng0594-29.
Chromosome sorting from hybrid cells offers enormous advantages for gene mapping and cloning, but purification of most chromosomes has been largely hindered by their similarity in size to other chromosomes. We have developed a novel cell line and strategy that allows simple, mass purification of mammalian chromosomes, permitting significant target genome enrichment. This strategy takes advantage of the small number of giant chromosomes (1,2,X) of the female Indian muntjac, a barking deer, avoiding the problem of size similarity. We introduced human chromosomes into a cell line derived from a muntjac and purified them to homogeneity using a relatively simple technique. This strategy should facilitate the isolation of chromosomes from species other than human for which hybrid cells are not available currently.
从杂交细胞中进行染色体分选为基因定位和克隆提供了巨大优势，但大多数染色体的纯化在很大程度上受到其与其他染色体大小相似性的阻碍。我们开发了一种新型细胞系和策略，可实现哺乳动物染色体的简单、大规模纯化，从而显著富集目标基因组。该策略利用了雌性印度麂（一种吠鹿）的少量巨大染色体（1、2、X），避免了大小相似性的问题。我们将人类染色体导入源自麂的细胞系，并使用相对简单的技术将它们纯化至同质状态。该策略应有助于从目前无法获得杂交细胞的人类以外的物种中分离染色体。
