Parreira V R, Arns C W, Yano T
Departamento de Microbiologia e Imunologia, Universidade Estadual de Campinas, São Paulo, Brazil.
FEMS Microbiol Lett. 1994 Jul 15;120(3):303-6. doi: 10.1111/j.1574-6968.1994.tb07050.x.
An agar overlay method, with Vero and HeLa cells, was used for detection of heat-labile enterotoxin and verotoxin from Escherichia coli. The method is more sensitive than the conventional cell culture assay, is rapid, easy to perform, and is suitable for epidemiological studies.
采用琼脂覆盖法,以Vero细胞和HeLa细胞检测大肠杆菌的不耐热肠毒素和维罗毒素。该方法比传统的细胞培养检测法更灵敏,速度快、操作简便,适用于流行病学研究。