Morawetz R, Mischak H, Goodnight J, Lendenfeld T, Mushinsky J F, Kubicek C P
Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Austria.
Gene. 1994 Sep 2;146(2):309-10. doi: 10.1016/0378-1119(94)90311-5.
A gene (pkt1) was isolated from the filamentous fungus Trichoderma reesei, which exhibits high homology with the yeast YPK1 and YKR2 (YPK2) genes. It contains a 2123-bp ORF that is interrupted by two introns, and it encodes a 662-amino-acid protein with a calculated M(r) of 72,820. During active growth, pkt1 is expressed as two mRNAs of 3.1 and 2.8 kb which differ in the 3' untranslated region due to the use of two different polyadenylation sites.
从丝状真菌里氏木霉中分离出一个基因（pkt1），它与酵母YPK1和YKR2（YPK2）基因具有高度同源性。它包含一个2123 bp的开放阅读框，该开放阅读框被两个内含子中断，并且编码一个662个氨基酸的蛋白质，计算所得的分子量为72,820。在活跃生长期间，pkt1以3.1 kb和2.8 kb的两种mRNA形式表达，由于使用了两个不同的聚腺苷酸化位点，这两种mRNA在3'非翻译区有所不同。
