Nakari T, Alatalo E, Penttilä M E
VTT Biotechnical Laboratory, Espoo, Finland.
Gene. 1993 Dec 22;136(1-2):313-8. doi: 10.1016/0378-1119(93)90486-m.
Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1 alpha (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.
从丝状真菌里氏木霉中分离出在含葡萄糖培养基上高表达的基因。用葡萄糖培养的真菌制备了一个cDNA文库，用相同的cDNA作为探针筛选该文库，并分离出信号最强的克隆。这导致了以前未鉴定基因的分离。其中五个基因代表最丰富的转录本，占总mRNA群体的1-3%，并且明显比里氏木霉的磷酸甘油酸激酶编码基因（pgk1）表达量更高。基于序列同源性，其中一个基因被鉴定为tef1，编码翻译延伸因子1α（TEF）。里氏木霉TEF与总状毛霉TEF3关系最为密切，整体氨基酸相似性为85%。有趣的是，里氏木霉tef1中似乎只存在一个2bp的外显子，它包含甘氨酸15密码子的前2bp。
