Avila-Cariño J, Lewin N, Yamamoto K, Tomita Y, Mellstedt H, Brodin B, Rosén A, Klein E
Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Stockholm, Sweden.
Int J Cancer. 1994 Sep 1;58(5):678-85. doi: 10.1002/ijc.2910580511.
Epstein-Barr virus (EBV)-carrying immortalized lymphoblastoid cell lines (LCLs) stimulate autologous T lymphocytes in vitro. This T-cell response is independent of the EBV-specific cellular memory because it also occurs in experiments with cells of seronegative individuals. The question can be posed whether the T-cell-stimulatory potential of the LCL is coupled to its immortalized state. B-CLL cells were exploited to study this question because the majority of clones, represented by different patients, can be infected with EBV but they rarely become immortalized. We have investigated the phenotypic changes and the T-cell-stimulatory capacity of EBV-infected B-CLL cells. One aliquot of CLL cells was infected with EBV, another was activated with a mixture of Staphylococcus aureus (SAC), IL-2 and the supernatant from the T-cell hybridoma MP6 (activation mixture, AcMx) and the third aliquot received both treatments. In accordance with the individual features of the clonal populations represented by each patient, the immunophenotypic changes imposed by these treatments differed. With the samples of 3 patients the allo-stimulatory potential showed the following ranking order: EBV and AcMx-treated cells > AcMx-treated > EBV-infected. An analysis of several activation-related surface markers and adhesion molecules on the cells did not reveal any association between their expression and the EBV-imposed potentiation of allostimulatory capacity. These results may be extrapolated to EBV-genome-carrying normal B cells, suggesting that they can persist in vivo only as long as they have the resting phenotype. Once they are activated, these cells may be recognized and eliminated by T lymphocytes.
携带爱泼斯坦-巴尔病毒（EBV）的永生化淋巴母细胞系（LCLs）在体外可刺激自体T淋巴细胞。这种T细胞反应不依赖于EBV特异性细胞记忆，因为在血清阴性个体的细胞实验中也会发生这种反应。可以提出这样一个问题，即LCL的T细胞刺激潜能是否与其永生化状态相关。利用B-CLL细胞来研究这个问题，因为以不同患者为代表的大多数克隆可被EBV感染，但它们很少会永生化。我们研究了EBV感染的B-CLL细胞的表型变化和T细胞刺激能力。将一份CLL细胞用EBV感染，另一份用金黄色葡萄球菌（SAC）、白细胞介素-2和T细胞杂交瘤MP6的上清液混合物（激活混合物，AcMx）激活，第三份细胞同时接受这两种处理。根据每位患者所代表的克隆群体的个体特征，这些处理所引起的免疫表型变化有所不同。对于3例患者的样本，同种异体刺激潜能呈现以下排序：经EBV和AcMx处理的细胞＞经AcMx处理的细胞＞经EBV感染的细胞。对细胞上几种与激活相关的表面标志物和黏附分子的分析未发现它们的表达与EBV增强的同种异体刺激能力之间存在任何关联。这些结果可外推至携带EBV基因组的正常B细胞，表明它们在体内只有保持静止表型时才能持续存在。一旦它们被激活，这些细胞可能会被T淋巴细胞识别并清除。
