Avila-Cariño J, Lewin N, Tomita Y, Szeles A, Sandlund A, Mosolits S, Mellstedt H, Klein G, Klein E
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Int J Cancer. 1997 Jan 6;70(1):1-8. doi: 10.1002/(sici)1097-0215(19970106)70:1<1::aid-ijc1>3.0.co;2-1.
In studies concerning the interaction of B-CLL cells and Epstein-Barr virus (EBV), we encountered one patient whose cells had several unusual properties. In addition to the B-cell markers, the CLL cells expressed the exclusive T-cell markers CD3 and CD8 and carried a translocation t(18,22)(q21;q11), involving the bcl-2 and Ig lambda loci. The patient represents the 4th reported CLL case with this translocation. The CLL cells could be infected and immortalized by the indigenous and by the prototype B958 virus in vitro. The T-cell markers were not detectable on the established lines. In all experiments the immortalized lines originated from the CLL cells. Their preferential emergence over virus-infected normal B cells may be coupled to the high expression of the bcl-2 gene due to the translocation. In spite of the sensitivity of CLL cells to EBV infection in vitro, no EBNA-positive cells were detected in the ex vivo population. In vitro, we could generate cytotoxic function in T-lymphocyte cultures which acted on autologous EBV-infected CLL cells. Therefore we assume that if such cells emerged in vivo they were eliminated by the T-cell response.
在有关B细胞慢性淋巴细胞白血病（B-CLL）细胞与爱泼斯坦-巴尔病毒（EBV）相互作用的研究中，我们遇到了一名患者，其细胞具有一些不寻常的特性。除了B细胞标志物外，慢性淋巴细胞白血病细胞还表达了独特的T细胞标志物CD3和CD8，并携带涉及bcl-2和Igλ基因座的t(18,22)(q21;q11)易位。该患者是第4例报道的具有这种易位的慢性淋巴细胞白血病病例。慢性淋巴细胞白血病细胞在体外可被本地病毒和原型B958病毒感染并永生化。在建立的细胞系上未检测到T细胞标志物。在所有实验中，永生化细胞系均源自慢性淋巴细胞白血病细胞。由于易位导致bcl-2基因的高表达，它们比病毒感染的正常B细胞更易出现。尽管慢性淋巴细胞白血病细胞在体外对EBV感染敏感，但在体外培养的细胞群体中未检测到EBNA阳性细胞。在体外，我们可以在作用于自体EBV感染的慢性淋巴细胞白血病细胞的T淋巴细胞培养物中产生细胞毒性功能。因此我们推测，如果此类细胞在体内出现，它们会被T细胞反应清除。
