Vanrompay D, Van Nerom A, Ducatelle R, Haesebrouck F
Department of Avian Diseases, State University of Ghent, Belgium.
J Clin Microbiol. 1994 Jun;32(6):1470-4. doi: 10.1128/jcm.32.6.1470-1474.1994.
Five commercially available immunoassays were evaluated for the detection of Chlamydia psittaci in cloacal and conjunctival swabs from industrially raised turkeys: IMAGEN (DAKO Diagnostics, Ely, Cambridgeshire, United Kingdom), Chlamydia CEL-VET IF (Cellabs, Brookvale, Australia), IDEIA (DAKO Diagnostics), CELISA (Cellabs), and CLEARVIEW (Unipath, Bedford, United Kingdom). Results were compared with isolation in Buffalo Green Monkey cells as a reference method. For the conjunctival samples, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 66, 0, 0, and 0%, respectively, as compared to the reference test. Also for the conjunctival samples, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, and the IDEIA were found to be 100, 11, and 92.8%, respectively. For the cloacal specimens, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 93.3, 26.6, 0, and 53.3%, respectively. Also for the cloacal specimens, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, and the CLEARVIEW test were found to be 92, 12, 100, and 88%, respectively. The IMAGEN test was the most sensitive and specific direct chlamydia antigen detection test for cloacal and conjunctival samples from turkeys.
对五种市售免疫测定法进行了评估，以检测工业化养殖火鸡泄殖腔和结膜拭子中的鹦鹉热衣原体：IMAGEN（英国剑桥郡伊利市达科诊断公司）、衣原体CEL-VET IF（澳大利亚布鲁克维尔市Cellabs公司）、IDEIA（英国剑桥郡伊利市达科诊断公司）、CELISA（澳大利亚布鲁克维尔市Cellabs公司）和CLEARVIEW（英国贝德福德市Unipath公司）。将结果与在布法罗绿猴细胞中进行分离培养的参考方法进行比较。对于结膜样本，与参考测试相比，IMAGEN测试、衣原体CEL-VET IF测试、IDEIA、CELISA和CLEARVIEW测试的灵敏度分别为100%、66%、0%、0%和0%。同样对于结膜样本，IMAGEN测试、衣原体CEL-VET IF测试和IDEIA的特异性分别为100%、11%和92.8%。对于泄殖腔标本，IMAGEN测试、衣原体CEL-VET IF测试、IDEIA、CELISA和CLEARVIEW测试的灵敏度分别为100%、93.3%、26.6%、0%和53.3%。同样对于泄殖腔标本，IMAGEN测试、衣原体CEL-VET IF测试、IDEIA和CLEARVIEW测试的特异性分别为92%、12%、100%和88%。IMAGEN测试是检测火鸡泄殖腔和结膜样本中衣原体抗原最灵敏和特异的直接检测方法。
