Kirkley B A, Easley K A, Washington J A
Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195-5140.
J Clin Microbiol. 1994 Jun;32(6):1547-9. doi: 10.1128/jcm.32.6.1547-1549.1994.
A controlled clinical evaluation comparing the Isolator system (Wampole Laboratories, Cranbury, N.J.) and the ESP 80A blood culture bottle in the automated ESP system (Difco Laboratories, Detroit, Mich.) was performed with 10,535 blood culture sets from patients with suspected septicemia. Of 1,150 positive cultures, 844 positive cultures from 285 patients with 394 septic episodes fulfilled the study criteria for minimum blood sample requirements in each system and clinical significance of isolates. The Isolator system detected statistically significantly more positive cultures of Staphylococcus aureus (P < 0.001), Enterococcus spp. (P = 0.007), Escherichia coli (P = 0.001), Alcaligenes xylosoxidans (P = 0.02), Xanthomonas maltophilia (P = 0.01), Candida albicans (P < 0.001), and Candida glabrata (P = 0.05). The Isolator system detected significantly more septic episodes due to S. aureus (P < 0.001), X. maltophilia (P = 0.02), and C. albicans (P = 0.004) than did the ESP 80A bottle; however, the two systems did not otherwise significantly differ in their abilities to detect septic episodes due to other organisms.
对疑似败血症患者的10535份血培养样本进行了一项对照临床评估，比较了隔离器系统（新泽西州克兰伯里的Wampole实验室）和自动化ESP系统（密歇根州底特律的Difco实验室）中的ESP 80A血培养瓶。在1150份阳性培养物中，来自285例患者、394次败血症发作的844份阳性培养物符合每个系统的最低血样要求和分离株临床意义的研究标准。隔离器系统在统计学上检测到显著更多的金黄色葡萄球菌阳性培养物（P < 0.001）、肠球菌属（P = 0.007）、大肠杆菌（P = 0.001）、木糖氧化产碱杆菌（P = 0.02）、嗜麦芽窄食单胞菌（P = 0.01）、白色念珠菌（P < 0.001）和光滑念珠菌（P = 0.05）。与ESP 80A瓶相比，隔离器系统检测到的由金黄色葡萄球菌（P < 0.001）、嗜麦芽窄食单胞菌（P = 0.02）和白色念珠菌（P = 0.004）引起的败血症发作显著更多；然而，在检测其他生物体引起的败血症发作方面，这两个系统在其他方面没有显著差异。
