Lundborg G, Zhao Q, Kanje M, Danielsen N, Kerns J M
Department of Hand Surgery, Malmö General Hospital, Sweden.
J Hand Surg Br. 1994 Jun;19(3):277-82. doi: 10.1016/0266-7681(94)90069-8.
The possibility that collateral sprouting could occur from intact axons in an undamaged sciatic nerve was studied in the rat by suturing either a 7-day predegenerated or a fresh nerve segment in an end-to-side fashion to the sciatic nerve proper. Following a 14- or 35-day recovery period, the pinch reflex test was performed on the transplanted segment to demonstrate the presence of sensory axons. The majority of cases, using a predegenerated nerve segment but not a fresh segment, responded positively. Neurofilament staining and histological examination confirmed the presence of axons in the attached nerve segment. In another series of experiments, the proximal peroneal fascicle was ligated and cut. Following a 7-day predegeneration period the distal stump was sutured end-to-side to the ipsilateral tibial fascicle. After 90 days, stimulation of the tibial nerve proximal to the attached site induced substantial contraction in both the native gastrocnemius muscle and the foreign tibialis anterior muscle. These findings suggest that collateral sprouting may occur from intact axons, perhaps induced by factors emanating from the attached nerve segment, and subsequently make functional peripheral connections.
通过将一段7天前已退变的或新鲜的神经段以端侧方式缝合到大鼠正常的坐骨神经上，研究了未受损的坐骨神经中完整轴突发生侧支发芽的可能性。在14天或35天的恢复期后，对移植段进行捏反射试验，以证明感觉轴突的存在。在大多数情况下，使用退变的神经段而非新鲜神经段时反应呈阳性。神经丝染色和组织学检查证实了附着神经段中存在轴突。在另一系列实验中，结扎并切断腓总神经近端束。经过7天的退变期后，将远端残端以端侧方式缝合到同侧胫神经束上。90天后，刺激附着部位近端的胫神经会引起原腓肠肌和异体胫前肌的显著收缩。这些发现表明，侧支发芽可能发生于完整轴突，可能是由附着神经段释放的因子诱导，随后建立功能性外周连接。
