Xu L Z, Larzul D, Delaporte E, Bréchot C, Kremsdorf D
Hybridotest, Institut Pasteur, Paris, France.
J Gen Virol. 1994 Sep;75 ( Pt 9):2393-8. doi: 10.1099/0022-1317-75-9-2393.
Following a survey of hepatitis C virus (HCV) infection recently carried in central Africa (Gabon), we cloned and sequenced PCR products of the 5' non-coding and capsid-encoding regions of HCV RNA from three randomly selected HCV RNA-positive Gabonese subjects. In the capsid-encoding region, the identity between the three Gabonese isolates was 91 to 98%. The three Gabonese sequences showed a divergence of 11 to 17% from published HCV genotypes I to IV (1a, 1b, 2a and 2b) isolates and of 6 to 11% from HCV genotype 4 isolates. Thus the Gabonese isolates, termed HC-G, belong to HCV genotype 4. Based on the sequences of the three isolates, a specific probe (cpsG) was designed to detect the HC-G genotype in 30 randomly selected anti-HCV-positive Gabonese subjects, 14 of whom were HCV RNA-positive. Analysis with cpsG showed that 10 of 14 of the HCV RNA-positive subjects were infected by the HC-G genotype. HC-G is therefore highly prevalent in the HCV RNA-positive Gabonese population. The availability of these Gabonese sequences should facilitate the design of specific serological tests for African HCV isolates.
在最近对中非（加蓬）进行的丙型肝炎病毒（HCV）感染调查之后，我们从三名随机挑选的HCV RNA阳性加蓬受试者中克隆并测序了HCV RNA 5'非编码区和衣壳编码区的PCR产物。在衣壳编码区，三个加蓬分离株之间的同源性为91%至98%。这三个加蓬序列与已发表的HCV基因I型至IV型（1a、1b、2a和2b）分离株的差异为11%至17%，与HCV基因4型分离株的差异为6%至11%。因此，这些被称为HC-G的加蓬分离株属于HCV基因4型。基于这三个分离株的序列，设计了一种特异性探针（cpsG），用于检测30名随机挑选的抗HCV阳性加蓬受试者中的HC-G基因型，其中14人HCV RNA呈阳性。用cpsG分析表明，14名HCV RNA阳性受试者中有10人感染了HC-G基因型。因此，HC-G在HCV RNA阳性的加蓬人群中高度流行。这些加蓬序列的获得应有助于为非洲HCV分离株设计特异性血清学检测方法。
