Malpezzi E L, Davino S C, Costa L V, Freitas J C, Giesbrecht A M, Roque N F
Departamento de Fisiologia Geral, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1994 Mar;27(3):749-54.
The hydroethanol extract of the roots of Petiveria alliacea L. (Phytolaccaceae) has been investigated previously as an antitumor agent against mouse Ehrlich ascites. The extract and its methanol, butanol and ether fractions exhibited an antimitotic effect on sea urchin egg development. The aqueous fraction did not produce inhibition of cell cleavage. At the first cleavage the inhibition, at the lowest concentration (10 micrograms/ml), produced by the ether fraction was 42%, whereas the inhibition produced by the total extract and by the other fractions was only 5 to 10% showing that the ether fraction was the most active. Even at higher concentrations the butanol and methanol fractions inhibit the cleavage about 30%. At the first cleavage, the ED50 of the hydroethanol extract and of the ether fraction were 45.02 and 12.40 micrograms/ml, respectively. Furthermore, in the second cleavage, the hydroethanol extract was about twice as potent as the methanol or butanol fractions (ED50 of 22.40, 44.80 and 54.10 micrograms/ml, respectively).
紫茉莉科紫茉莉属植物紫茉莉根的水乙醇提取物先前已作为抗小鼠艾氏腹水瘤剂进行过研究。该提取物及其甲醇、丁醇和乙醚馏分对海胆卵发育表现出抗有丝分裂作用。水相馏分未对细胞分裂产生抑制作用。在第一次分裂时，乙醚馏分在最低浓度（10微克/毫升）下产生的抑制率为42%，而总提取物和其他馏分产生的抑制率仅为5%至10%，这表明乙醚馏分活性最强。即使在较高浓度下，丁醇和甲醇馏分也能抑制约30%的分裂。在第一次分裂时，水乙醇提取物和乙醚馏分的半数有效剂量（ED50）分别为45.02和12.40微克/毫升。此外，在第二次分裂时，水乙醇提取物的效力约为甲醇或丁醇馏分的两倍（ED50分别为22.40、44.80和54.10微克/毫升）。
