Zhou Z, He L, Zhou J
Dept. of Neurobiology, Shanghai Medical University, China.
Zhen Ci Yan Jiu. 1993;18(1):14-20.
In this experiment, three groups were used to detect c-fos expression in the CNS in the awake male, S.D. rats, i.e, EA of "Quanliao" (14 Hz), electrostimulation of the rat tail (0.4-0.6 mA) and the control. Fos was identified by ABC immunohistochemical method. The results were as follows: I. The labelling cellular nuclei of pain group are in the laminae I, II, V, VII, and X of the spinal cord the medial part of the frontal cortex (FC) and that of the EA group were in the trigeminal nucleus and the lateral area of the FC. The number of the labelling nuclei (n) of EA group war more than that of the pain group in the rostral ventromedical medulla, the lateral reticular, the solitary n. the popiaqueductal grey, the parafacicular n, the centromedian n. and the subparafascicular n, of the thalamus. 2. The labelling nuclei of EA and pain groups appear commonly in the dorsal raphe n., the locus ceruleus, the periventricular n. of the thalamus, the hypothalamue, the lateral habenular n., and the septal area. In the control animals, in general, the labelled nuclei were sparsely seen.
在本实验中,使用三组清醒雄性SD大鼠来检测中枢神经系统(CNS)中的c-fos表达,即“颧髎”电针(14赫兹)、大鼠尾部电刺激(0.4 - 0.6毫安)和对照组。采用ABC免疫组织化学方法鉴定Fos。结果如下:1. 疼痛组标记的细胞核位于脊髓的I、II、V、VII和X层、额叶皮质(FC)内侧部分,而电针组的标记细胞核位于三叉神经核和FC外侧区域。在延髓头端腹内侧、外侧网状核、孤束核、导水管周围灰质、束旁核、中央中核和丘脑束旁下核中,电针组标记细胞核的数量(n)多于疼痛组。2. 电针组和疼痛组标记的细胞核常见于中缝背核、蓝斑、丘脑室周核、下丘脑、外侧缰核和隔区。在对照动物中,一般标记的细胞核较少见。