di Francesco P, Gaziano R, Casalinuovo I A, Belogi L, Palamara A T, Favalli C, Garaci E
Department of Experimental Medicine and Biochemical Sciences, University of Rome, Tor Vergata, Italy.
Clin Exp Immunol. 1994 Sep;97(3):347-52. doi: 10.1111/j.1365-2249.1994.tb06093.x.
Treatment of systemic infection with Candida albicans with a combination of an antifungal agent (i.e. fluconazole) and a thymus-derived immunostimulant (i.e. thymosin alpha 1 (T alpha 1)) in mice immunosuppressed by morphine treatments was investigated. In normal mice, fluconazole given after infection with 10(6) C. albicans cells was more effective than in mice treated with morphine. Combination treatment with fluconazole and T alpha 1 prolonged survival and reduced the fungal burden in the kidneys of immunosuppressed mice. We also investigated the influence of this combined treatment on killing properties of polymorphonuclear leucocytes (PMN) and natural killer (NK) cell activity, inhibited by morphine administrations. Treatment with T alpha 1 or fluconazole as single agents promoted a recovery of normal NK cell activity and intracellular killing of C. albicans by PMN, while the combination significantly increased both of these responses, probably through the modulation of lymphokine production. Our data suggest that the additive effect of T alpha 1 and fluconazole is due to a direct antifungal action and activation of the immunocompetence.
研究了在经吗啡处理免疫抑制的小鼠中，使用抗真菌剂（即氟康唑）和胸腺来源的免疫刺激剂（即胸腺肽α1（Tα1））联合治疗白色念珠菌全身性感染的情况。在正常小鼠中，感染10⁶个白色念珠菌细胞后给予氟康唑比给予吗啡处理的小鼠更有效。氟康唑和Tα1联合治疗可延长免疫抑制小鼠的生存期并减轻肾脏中的真菌负荷。我们还研究了这种联合治疗对被吗啡给药抑制的多形核白细胞（PMN）杀伤特性和自然杀伤（NK）细胞活性的影响。单独使用Tα1或氟康唑治疗可促进正常NK细胞活性的恢复以及PMN对白色念珠菌的细胞内杀伤，而联合治疗则可能通过调节淋巴因子的产生显著增强这两种反应。我们的数据表明，Tα1和氟康唑的相加作用是由于直接的抗真菌作用和免疫能力的激活。
