[RNA片段，用于DNA体外复制的必要引物]
[RNA-fragments, necessary primers for the in vitro replication of DNA].
作者信息
Beljanski M, Beljanski M, Plawecki M, Bourgarel P, Lépine P
出版信息
C R Acad Hebd Seances Acad Sci D. 1975 Jan 20;280(3):363-6.
PMID:808339
Abstract
Partial degradation of ribosomal RNAs (E. coli rich in purine nucleotides) by different ribonucleases gives rise to the appearance of several families of RNA-fragments which after separation on "Sephadex G 25" were analyzed for base ratio, size and biological activity. In the presence of DNA dependent DNA polymerase, RNA-fragments act as primers for in vitro replication of DNAs from numerous sources.
摘要
用不同的核糖核酸酶对核糖体RNA（富含嘌呤核苷酸的大肠杆菌）进行部分降解，会产生几个RNA片段家族。这些片段在“葡聚糖G 25”上分离后，对其碱基比例、大小和生物活性进行了分析。在依赖DNA的DNA聚合酶存在的情况下，RNA片段可作为来自多种来源的DNA进行体外复制的引物。
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