Imazato S, Torii M, Tsuchitani Y, McCabe J F, Russell R R
Department of Operative Dentistry, Osaka University Faculty of Dentistry, Japan.
J Dent Res. 1994 Aug;73(8):1437-43. doi: 10.1177/00220345940730080701.
Attempts to produce resin composite with antibacterial properties by incorporation of an antibacterial agent such as chlorhexidine have been reported, but problems can arise due to release of the inhibitory agent from the composite. Such problems may include toxic effects, influence on mechanical properties, and loss of effectiveness. A new monomer, methacryloyloxydodecylpyridinium bromide (MDPB), was synthesized by combining an antibacterial agent and methacryloyl group. The monomer was incorporated into resin composite to develop a non-releasing antibacterial composite. The ability of composite incorporating MDPB to inhibit growth and plaque accumulation by Streptococcus mutans in vitro was assayed, elution of antibacterial components from the material was investigated, and the influence of incorporation of MDPB on the mechanical properties of composite was studied. Uncured MDPB revealed antibacterial activity against S. mutans and six other species of oral streptococci, with the minimum inhibitory concentration for S. mutans being comparable with that of triclosan. After composite incorporating MDPB was cured, no elution of the antibacterial components was observed from the material, even after 90 days' immersion in water or other solvents. Growth of S. mutans on agar under specimens of MDPB-containing composite was inhibited compared with controls. In a bacterial accumulation study, S. mutans accumulated to a lesser degree on the surface of composite incorporating MDPB (p < 0.05) than on control. Incorporation of MDPB had no significant influence on the mechanical properties of the composite.
已有报道尝试通过加入抗菌剂如洗必泰来制备具有抗菌性能的树脂复合材料，但由于复合材料中抑制剂的释放可能会出现问题。这些问题可能包括毒性作用、对机械性能的影响以及有效性的丧失。一种新的单体，甲基丙烯酰氧基十二烷基溴化吡啶（MDPB），通过将抗菌剂与甲基丙烯酰基结合而合成。将该单体加入树脂复合材料中以开发一种不释放抗菌成分的复合材料。测定了含MDPB的复合材料在体外抑制变形链球菌生长和菌斑积聚的能力，研究了该材料抗菌成分的洗脱情况，并研究了MDPB的加入对复合材料机械性能的影响。未固化的MDPB对变形链球菌和其他六种口腔链球菌具有抗菌活性，变形链球菌的最低抑菌浓度与三氯生相当。含MDPB的复合材料固化后，即使在水中或其他溶剂中浸泡90天后，也未观察到抗菌成分的洗脱。与对照组相比，含MDPB复合材料标本下琼脂上变形链球菌的生长受到抑制。在细菌积聚研究中，变形链球菌在含MDPB复合材料表面的积聚程度低于对照组（p<0.05）。MDPB的加入对复合材料的机械性能没有显著影响。
