Ebi N, Imazato S, Noiri Y, Ebisu S
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Dent Mater. 2001 Nov;17(6):485-91. doi: 10.1016/s0109-5641(01)00006-9.
Previously, we have reported that incorporation of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) was effective in immobilizing bactericide in the resin matrix, and an antibacterial composite without release of the agent could be achieved. In this study, an attempt was made to increase the density of bactericide immobilized in composite, and the inhibitory effects of this modified antibacterial composite on plaque accumulation were determined, focusing on the reliability of the effects and the mechanisms to affect the plaque formation.
An experimental composite containing immobilized bactericide at 2.83% was prepared by the incorporation of MDPB into a prepolymerized resin filler of control composite, and elution of antibacterial components and inhibition of in vitro plaque accumulation by Streptococcus mutans were determined. The inhibitory effects of the experimental composite on the attachment, glucan synthesis and growth of S. mutans on the surface were also examined in addition to the comparison of surface roughness and hydrophobicity with controls. The results were analyzed using the Student's t-test.
The experimental composite had reproducible inhibitory effects against plaque accumulation compared with control (p<0.05), although it showed no elution of unpolymerized MDPB. The plaque-inhibitory effect of the experimental composite was found to depend upon the ability to inhibit the attachment, glucan synthesis, and growth of bacteria on its surface as no significant differences in the surface characteristics were obtained between control and experimental composites (p>0.05).
It was indicated that the experimental composite containing bactericide-immobilized filler has the possibility to be used clinically with an effective anti-plaque property.
此前，我们曾报道将抗菌单体12-甲基丙烯酰氧基十二烷基溴化吡啶（MDPB）掺入树脂基质中可有效固定杀菌剂，从而制备出不释放杀菌剂的抗菌复合材料。在本研究中，我们试图提高复合材料中固定化杀菌剂的密度，并确定这种改性抗菌复合材料对牙菌斑积聚的抑制作用，重点关注其作用效果的可靠性以及影响牙菌斑形成的机制。
通过将MDPB掺入对照复合材料的预聚合树脂填料中，制备出含2.83%固定化杀菌剂的实验性复合材料，并测定抗菌成分的洗脱情况以及对变形链球菌体外牙菌斑积聚的抑制作用。除了比较其与对照材料的表面粗糙度和疏水性外，还检测了实验性复合材料对变形链球菌在表面的附着、葡聚糖合成及生长的抑制作用。结果采用学生t检验进行分析。
与对照材料相比，实验性复合材料对牙菌斑积聚具有可重复的抑制作用（p<0.05），尽管未检测到未聚合的MDPB洗脱。实验性复合材料的牙菌斑抑制作用取决于其抑制细菌在表面附着、葡聚糖合成及生长的能力，因为对照复合材料与实验性复合材料在表面特性上未获得显著差异（p>0.05）。
结果表明，含固定化杀菌剂填料的实验性复合材料有可能作为具有有效防牙菌斑性能的材料应用于临床。
