Powers D C
Geriatric Research, Education and Clinical Center, St. Louis VAMC, MO 63125.
J Med Virol. 1994 May;43(1):57-61. doi: 10.1002/jmv.1890430111.
We previously reported an age-associated impairment of serum immunoglobulin G (IgG) antibody responses to inactivated influenza virus vaccine. The present study extends these observations by examining the IgG subclass distribution of vaccine responses measured by enzyme linked immunosorbent assay in healthy adults aged < 40 (young), 40-64 (middle-aged), and > or = 65 (elderly) years. Serological responses in all age groups showed antibodies that were predominantly IgG1 and secondarily IgG3. Influenza antigen-specific IgG4 titers did not change following vaccination, and antibodies of the IgG2 subclass were not detected in any serum specimens. Aging was associated with a significant impairment of IgG1, but not of IgG3, antibody production. Relative differences in the magnitude and frequency of response between IgG1 and IgG3 subclasses, which were present in young and middle-aged adults (viz., IgG1 > IgG3), were less apparent in the elderly. This observation was confirmed in a second analysis of IgG subclass-specific responses in a separate cohort of elderly vaccinees. These results suggest that the age-related impairment of humoral responses to inactivated influenza virus vaccine is primarily accounted for by differences in IgG1 antibody production, and that IgG3 antibodies make up a larger proportion of the overall serologic response in the elderly than they do in younger persons.
我们之前报道过，血清免疫球蛋白G（IgG）对灭活流感病毒疫苗的抗体反应存在与年龄相关的损害。本研究通过检测酶联免疫吸附测定法测得的健康成年人（年龄<40岁（年轻组）、40 - 64岁（中年组）和≥65岁（老年组））疫苗反应的IgG亚类分布，扩展了这些观察结果。所有年龄组的血清学反应显示，抗体主要为IgG1，其次为IgG3。接种疫苗后，流感抗原特异性IgG4滴度未发生变化，且在任何血清标本中均未检测到IgG2亚类抗体。衰老与IgG1抗体产生的显著损害相关，但与IgG3无关。年轻和中年成年人中存在的IgG1和IgG3亚类之间反应强度和频率的相对差异（即IgG1>IgG3）在老年人中不太明显。在另一组老年疫苗接种者中对IgG亚类特异性反应进行的第二次分析证实了这一观察结果。这些结果表明，与年龄相关的对灭活流感病毒疫苗体液反应的损害主要是由IgG1抗体产生的差异所致，并且IgG3抗体在老年人总体血清学反应中所占比例比在年轻人中更大。
