Manen J F, Savolainen V, Simon P
Conservatoire et Jardin Botaniques, Geneva, Switzerland.
J Mol Evol. 1994 Jun;38(6):577-82. doi: 10.1007/BF00175877.
The plastid atpB-rbcL intergene has been analyzed within a wide range of plants covering the major dicot lineages. New sequences from 13 plant species were determined and aligned with three already-known sequences. The promoters of the rbcL and the atpB genes were localized and analyzed according to published observations in spinach and tobacco. The evolutionary conservation of two atpB promoters, separated by 113-262 nucleotides, is strong support that both are functionally active, and it also allows a discrimination between the previously reported atpB transcripts. Moreover, the radically distinct sequences of the two atpB promoters suggest that they interact with two distinct initiation complexes. The alignment also confirms the much higher conservation of the leader sequence in the rbcL mRNA than in the atpB mRNA among dicots, presuming a function at the posttranscriptional level.
质体atpB-rbcL基因间隔区已在涵盖主要双子叶植物谱系的多种植物中进行了分析。测定了13种植物的新序列，并与三个已知序列进行了比对。根据菠菜和烟草中已发表的观察结果，定位并分析了rbcL和atpB基因的启动子。由113 - 262个核苷酸分隔的两个atpB启动子在进化上的保守性有力支持了两者均具有功能活性，这也使得能够区分先前报道的atpB转录本。此外，两个atpB启动子截然不同的序列表明它们与两种不同的起始复合物相互作用。比对还证实，在双子叶植物中，rbcL mRNA前导序列的保守性远高于atpB mRNA，推测其在转录后水平发挥作用。
