呼肠孤病毒分离株混合感染后的干扰与M2基因有关。

Interference following mixed infection of reovirus isolates is linked to the M2 gene.

作者信息

Rozinov M N, Fields B N

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

J Virol. 1994 Oct;68(10):6667-71. doi: 10.1128/JVI.68.10.6667-6671.1994.

DOI:10.1128/JVI.68.10.6667-6671.1994

PMID:8084000

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237087/

Abstract

Following infection by pairs of reovirus isolates consisting of combinations of reovirus T1 Lang, T2 Jones, or T3 Dearing, we found that one of the isolates interfered with the yield of progeny RNA derived from the other parents. The most significant interference was produced by T2 Jones or T3 Dearing, when mixed with T1 Lang. Genetic analysis revealed that the presence of the M2 gene in the interfering parent (in the T1 Lang x T3 Dearing pair) was linked to interference. Studies on interference in infected cells indicated that interference occurs after adsorption and penetration.

摘要

在用呼肠孤病毒T1 Lang、T2 Jones或T3 Dearing组合而成的成对呼肠孤病毒分离株感染后，我们发现其中一种分离株会干扰源自其他亲本的子代RNA的产量。当与T1 Lang混合时，T2 Jones或T3 Dearing产生的干扰最为显著。遗传分析表明，干扰亲本（在T1 Lang×T3 Dearing组合中）中M2基因的存在与干扰有关。对受感染细胞中干扰现象的研究表明，干扰发生在吸附和穿透之后。