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人免疫缺陷病毒1型包膜蛋白gp120在人胎儿星形胶质细胞上的新型结合位点的鉴定

Characterization of a novel binding site for the human immunodeficiency virus type 1 envelope protein gp120 on human fetal astrocytes.

作者信息

Ma M, Geiger J D, Nath A

机构信息

Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.

出版信息

J Virol. 1994 Oct;68(10):6824-8. doi: 10.1128/JVI.68.10.6824-6828.1994.

DOI:10.1128/JVI.68.10.6824-6828.1994
PMID:8084022
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237112/
Abstract

125I-labeled recombinant gp120 bound to primary cultures of human fetal astrocytes with a single class of 260-kDa binding sites, with a Kd of 26 nM and maximal number of binding sites of 29.9 fmol/4 x 10(4) cells. Neither CD4 nor galactocerebroside was detectable on astrocytes, and 125I-labeled recombinant gp120 binding to astrocytes was not blocked by antibodies against galactocerebroside or the gp120 binding domain of CD4.

摘要

125I标记的重组gp120与人类胎儿星形胶质细胞原代培养物结合，具有一类260 kDa的结合位点，解离常数（Kd）为26 nM，最大结合位点数为29.9 fmol/4×10⁴个细胞。星形胶质细胞上未检测到CD4或半乳糖脑苷脂，且抗半乳糖脑苷脂或CD4的gp120结合域的抗体不能阻断125I标记的重组gp120与星形胶质细胞的结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3244/237112/e4196db31a72/jvirol00019-0703-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3244/237112/365ccce0f2a2/jvirol00019-0702-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3244/237112/e4196db31a72/jvirol00019-0703-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3244/237112/365ccce0f2a2/jvirol00019-0702-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3244/237112/e4196db31a72/jvirol00019-0703-a.jpg

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