Maxwell L, Collecutt T, Gledhill M, Sharma S, Edgar S, Gavin J B
Department of Pathology, University of Auckland School of Medicine, New Zealand.
Ultrasound Med Biol. 1994;20(4):383-90. doi: 10.1016/0301-5629(94)90007-8.
To determine the effects of exposure of endothelial cells to therapeutic ultrasound on leucocyte adhesion to these cells, confluent cultures of bovine aortic endothelial cells in tissue culture flasks and on collagen coated coverslips were exposed to a maximum of 1.6 W/cm2 1 MHz continuous ultrasound for 15 min, then incubated with neutrophil-rich suspensions containing a known number of viable human leucocytes for intervals from 1 to 240 min. After incubation, nonadherent cells were decanted, the number of leucocytes in the eluted suspension determined and the percentage of adhered cells calculated. Endothelial cell viability was not significantly reduced by ultrasound or by incubation with leucocytes. Whereas untreated endothelial cells were flattened and had surface microvilli, those exposed to ultrasound were plump, rounded and more widely separated along their borders. The percentage of leucocytes that adhered to sonicated endothelium was significantly higher, and this proportion increased with the duration of incubation. Thus, exposure of endothelial cells to ultrasound in vitro can cause intercellular separation and increase the adhesion to leucocytes. If such changes were also to occur in vivo, therapeutic ultrasound would potentiate the inflammatory response.
为了确定内皮细胞暴露于治疗性超声下对白细胞黏附于这些细胞的影响，将组织培养瓶中以及胶原包被盖玻片上的牛主动脉内皮细胞汇合培养物暴露于最大强度为1.6 W/cm²的1 MHz连续超声下15分钟，然后与含有已知数量活的人类白细胞的富含中性粒细胞的悬液孵育1至240分钟。孵育后，倾析出未黏附的细胞，测定洗脱悬液中的白细胞数量，并计算黏附细胞的百分比。超声处理或与白细胞孵育均未显著降低内皮细胞的活力。未处理的内皮细胞扁平且有表面微绒毛，而暴露于超声下的内皮细胞则饱满、呈圆形且沿其边界分隔更宽。黏附于经超声处理的内皮细胞的白细胞百分比显著更高，且该比例随孵育时间延长而增加。因此，体外将内皮细胞暴露于超声可导致细胞间分离并增加对白细胞的黏附。如果体内也发生此类变化，治疗性超声将增强炎症反应。
