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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Robinson I M, Kinnick R R, Greenleaf J F, Fernandez J M

Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905, USA.

J Physiol. 1996 Apr 1;492 ( Pt 1)(Pt 1):257-63. doi: 10.1113/jphysiol.1996.sp021306.

In this study the secretory response of individual bovine adrenal chromaffin cells was monitored using amperometric carbon-fibre microelectrodes. Cells were stimulated to secrete by exposure to 20-100 microseconds long tonebursts of ultrasound (2-4 x 10(5) Pa; peak pressure at 1 MHz). 2. Three types of secretory responses were observed: an almost instantaneous response, a delayed release of catecholamines, or a series of 'burst-like' secretory bouts. 3. Fura-2 measurements of intracellular Ca2+ concentrations showed that the release of catecholamines was accompanied by an increase in the intracellular Ca2+ concentration. In the absence of extracellular Ca2+, secretory responses were not evoked showing that Ca2+ entry was necessary to elicit catecholamine release.

在本研究中，使用安培碳纤维微电极监测单个牛肾上腺嗜铬细胞的分泌反应。通过暴露于20 - 100微秒长的超声音调脉冲（2 - 4×10⁵帕；1兆赫兹时的峰值压力）刺激细胞分泌。2. 观察到三种类型的分泌反应：几乎瞬间的反应、儿茶酚胺的延迟释放或一系列“爆发样”分泌发作。3. 用Fura - 2测量细胞内Ca²⁺浓度表明，儿茶酚胺的释放伴随着细胞内Ca²⁺浓度的增加。在没有细胞外Ca²⁺的情况下，不会引发分泌反应，这表明Ca²⁺内流是引发儿茶酚胺释放所必需的。

Robinson I M, Kinnick R R, Greenleaf J F, Fernandez J M

Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905, USA.

J Physiol. 1996 Apr 1;492 ( Pt 1)(Pt 1):257-63. doi: 10.1113/jphysiol.1996.sp021306.

In this study the secretory response of individual bovine adrenal chromaffin cells was monitored using amperometric carbon-fibre microelectrodes. Cells were stimulated to secrete by exposure to 20-100 microseconds long tonebursts of ultrasound (2-4 x 10(5) Pa; peak pressure at 1 MHz). 2. Three types of secretory responses were observed: an almost instantaneous response, a delayed release of catecholamines, or a series of 'burst-like' secretory bouts. 3. Fura-2 measurements of intracellular Ca2+ concentrations showed that the release of catecholamines was accompanied by an increase in the intracellular Ca2+ concentration. In the absence of extracellular Ca2+, secretory responses were not evoked showing that Ca2+ entry was necessary to elicit catecholamine release.

在本研究中，使用安培碳纤维微电极监测单个牛肾上腺嗜铬细胞的分泌反应。通过暴露于20 - 100微秒长的超声音调脉冲（2 - 4×10⁵帕；1兆赫兹时的峰值压力）刺激细胞分泌。2. 观察到三种类型的分泌反应：几乎瞬间的反应、儿茶酚胺的延迟释放或一系列“爆发样”分泌发作。3. 用Fura - 2测量细胞内Ca²⁺浓度表明，儿茶酚胺的释放伴随着细胞内Ca²⁺浓度的增加。在没有细胞外Ca²⁺的情况下，不会引发分泌反应，这表明Ca²⁺内流是引发儿茶酚胺释放所必需的。