枯草芽孢杆菌糖化型α-淀粉酶催化麦芽糖水解速率对底物浓度的依赖性
Substrate concentration dependence of the rate of maltose hydrolysis by saccharifying alpha-amylase from B. subtilis.
作者信息
Shibaoka T, Inatani T, Hiromi K, Watanabe T
出版信息
J Biochem. 1975 May;77(5):965-8. doi: 10.1093/oxfordjournals.jbchem.a130821.
Abstract
- The action of saccharifying alpha-amylase from B. subtilis [EC 3.2.1.1] on eq- (the equilibrium mixture of alpha- and beta-forms) and beta-maltose was studied at pH 5.4 and 25 degrees. 2. The plots of initial velocity versus substrate concentration showed remarkable sigmoidal curves for both substrates. 3. The value of Hill's coefficient for eq- and beta-maltose were determined to be 3.3 and 3.8, respectively.
摘要
- 在pH 5.4和25摄氏度条件下，研究了枯草芽孢杆菌[EC 3.2.1.1]的糖化α-淀粉酶对α-和β-型平衡混合物（eq-）以及β-麦芽糖的作用。2. 初始速度与底物浓度的关系图显示，两种底物均呈现出明显的S形曲线。3. 确定eq-和β-麦芽糖的希尔系数分别为3.3和3.8。
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