Terzin A L, Mudrić V, Vuković B
Microbios. 1975;12(47-48):83-9.
Sera (1,333) were collected from patients at various days after the onset of manifest B-virus hepatitis; from long term asymptomatic carriers of Au-antigen, with or without a demonstrable history of hepatitis; and from apparently healthy Au-negative persons. Au-anti-genaemia was tested with gel-diffusion (GD), counter immunoelectrophoresis (CIE), reversed haemagglutination (RHA) and complement fixation (CF). The CF test was found to be as sensitive as the RHA test, about 10 times more sensitive than the CIE, and about 25 times more sensitive than the GD test. With greater amounts of antibodies used in CF tests, the titres of AU-antigen were higher and the heights of prozones were lower. It is stressed that in screening for Au-antigen, sera should be tested in dilutions from 2 to 64, against 4 to 8 CF units of antibody. The frequency of asymptomatic long term Au-carriers, in the group with a known history of hepatitis, amounted to 41%, and themean titre of Au-antigen was 1:392. In the group of long-term carriers with no detectable history of hepatitis, the rate of Au-carriers was 1.6%, and the mean titre was 1:19.
收集了1333份血清样本，这些样本来自显性B型病毒性肝炎发病后不同时间的患者；来自澳抗长期无症状携带者，这些携带者有或没有可证实的肝炎病史；以及来自表面健康的澳抗阴性者。采用凝胶扩散法（GD）、对流免疫电泳法（CIE）、反向血凝法（RHA）和补体结合法（CF）检测澳抗血症。发现补体结合试验与反向血凝试验一样灵敏，比对流免疫电泳法灵敏约10倍，比凝胶扩散法灵敏约25倍。在补体结合试验中使用更多量抗体时，澳抗的效价更高，前带高度更低。强调在筛查澳抗时，血清应以2至64倍稀释度进行检测，对抗4至8个补体结合单位的抗体。在有肝炎已知病史的组中，无症状长期澳抗携带者的频率为41%，澳抗的平均效价为1:392。在无可检测肝炎病史的长期携带者组中，澳抗携带者的比例为1.6%，平均效价为1:19。
